Syphilis Elisa

Oscar Syphilis ELISA is intended used for qualitative detection of antibodies of Treponema pallidum in human serum / plasma. Kit is available in 96T pack. Microwell strips are coated with recombinant antigens of syphilis. Samples along with positive and negative controls are added in the coated wells and incubated. The wells are washed to remove unbound components and syphilis recombinant antigens conjugated to Horse Reddish Peroxidase are added. After a short incubation the wells are washed again and bound enzyme is detected by adding substrate. The reaction is stopped after specified time with acid and absorbance is determined for each well at 450 nm with an ELISA reader. The cut-off value is calculated by the given formula and absorbance of all the wells are compared with the cut-off value. Any sample having absorbance more than the cut-off value is considered reactive.