HCV Elisa

Oscar HCV ELISA detects antibody of HCV (Hepatitis C Virus) in human serum / plasma. It is available in 96T pack. HCV ELISA is a solid phase Enzyme Linked Immunosorbent Assay (ELISA) which employs highly purified recombinant antigen representing most conserved antigenic segments of Core, NS3, NS4 and NS5 antigens. Microwell strips are coated with recombinant HCV antigen. Diluted samples along with positive and negative controls are added in the coated wells and incubated. The wells are washed to remove unbound components and goat antihuman IgG conjugated to Horse Reddish Peroxidase is added. After a short incubation the wells are washed again, and bound enzyme is detected by adding substrate. The reaction is stopped after specified time with acid and absorbance is determined for each well at 450 nm with an ELISA reader. The cutoff value is calculated by the given formula and absorbance of all the wells are compared with the cutoff value. Any sample having absorbance more than the cutoff value is considered reactive.