The primer and probe mix adopts the dual-target gene design, which targets the specific conserved
sequence encoding the ORF 1ab gene and the nucleoprotein N gene. With the PCR reaction mix
provided, the amplification of template can be quantitatively monitored by the increasing
fluorescence signal detected by a real time PCR instrument.
The PCR detection system includes an endogenous internal control primer and probe mix. The result
of internal control provides the accuracy of sampling and extraction process, in order to avoid false
negative results.